This question has been asked a couple of times in SO and many times in other sites. But I didn't get any satisfiable answer.
My problem:
I have a java web application which uses simple JDBC to connect to mysql database through Glassfish application server.  
I have used connection pooling in glassfish server with the following configurations:
Initial Pool Size: 25
Maximum Pool Size: 100
Pool Resize Quantity: 2
Idle Timeout: 300 seconds
Max Wait Time: 60,000 milliseconds  
The application has been deployed for last 3 months and it was running flawlessly too.
But from last 2 days the following error is coming at the time of login.  
Partial StackTrace
com.mysql.jdbc.exceptions.MySQLNonTransientConnectionException: No operations allowed after connection closed.Connection was implicitly closed due to underlying exception/error:  
** BEGIN NESTED EXCEPTION **  
com.mysql.jdbc.CommunicationsException  
MESSAGE: Communications link failure due to underlying exception:  
** BEGIN NESTED EXCEPTION **  
java.io.EOFException  
MESSAGE: Can not read response from server. Expected to read 4 bytes, read 0 bytes before connection was unexpectedly lost.  
STACKTRACE:  
java.io.EOFException: Can not read response from server. Expected to read 4 bytes, read 0 bytes before connection was unexpectedly lost.  
at com.mysql.jdbc.MysqlIO.readFully(MysqlIO.java:1997)  
at com.mysql.jdbc.MysqlIO.reuseAndReadPacket(MysqlIO.java:2411)  
at com.mysql.jdbc.MysqlIO.checkErrorPacket(MysqlIO.java:2916)  
at com.mysql.jdbc.MysqlIO.sendCommand(MysqlIO.java:1631)  
at com.mysql.jdbc.MysqlIO.sqlQueryDirect(MysqlIO.java:1723)  
at com.mysql.jdbc.Connection.execSQL(Connection.java:3256)  
at com.mysql.jdbc.PreparedStatement.executeInternal(PreparedStatement.java:1313)  
at com.mysql.jdbc.PreparedStatement.executeQuery(PreparedStatement.java:1448)  
............  
............  
my application traces....  
What caused this error suddenly ? I have lost a lot of time for this.
EDIT : The problem even persists after restarting the server. As per DBA two of the important mysql server configurations are:
wait_timeout : 1800 seconds
connect_timeout : 10 seconds
NOTE : Other applications deployed in the same server connecting to the same database and using different pools are running smoothly.  
EDIT-2 : After reading a lot of things and expecting some positive outcome I made these changes to my connection pool.
Max Wait Time : 0 (previously it was 60 seconds)
Connection Validation : Required
Validation Method : table
Table Name : Demo
Validate Atmost Once : 40 seconds
Creation Retry Attempts : 1
Retry Intervals : 5 seconds
Max Connection Usage : 5  
And this worked as the application is running for 3 days consistently. But I got a very strange and interesting result of out this. While monitoring the connection pool, I found these figures:
NumConnAcquired : 44919 Count
NumConnReleased : 44919 Count
NumConnCreated : 9748 Count
NumConnDestroyed : 9793 Count
NumConnFailedValidation : 70 Count
NumConnFree : 161 Count
NumConnUsed : -136 Count  
How can the NumConnFree become 161 as I have Maximum Pool Size = 100 ?
How can the NumConnUsed become -136, a negative number ?
How can the NumConnDestroyed > NumConnCreated ? 
 
     
     
     
     
     
     
     
     
     
     
     
     
     
    