You can use a combination of colClasses with "NULL" entries to "blank-out" the commas (also still needing , fill=TRUE:
read.table(text="1,2,3,4,5,6,7,8,,,,,,,,,,,,,,,,,,
 9,9,9,9,9,9,9,9,,,,,,,,,,,,,,,,,", sep=",", fill=TRUE, colClasses=c(rep("numeric", 8), rep("NULL", 30)) )
#------------------
  V1 V2 V3 V4 V5 V6 V7 V8
1  1  2  3  4  5  6  7  8
2  9  9  9  9  9  9  9  9
Warning message:
In read.table(text = "1,2,3,4,5,6,7,8,,,,,,,,,,,,,,,,,,\n9,9,9,9,9,9,9,9,,,,,,,,,,,,,,,,,",  :
  cols = 26 != length(data) = 38
I needed to add back in the missing linefeed at the end of the first line. (Yet another reason why you should edit questions rather than putting data examples in the comments.) There was an octothorpe in the header which required the comment.char be set to "":
read.table(text="Document_Name,Sequence_Name,Track_Name,Type,Name,Sequence,Minimum,Min_(with_gaps),Maximum,Max_(with_gaps),Length,Length_(with_gaps),#_Intervals,Direction,Average_Quality,Coverage,modified_by,Polymorphism_Type,Strand-Bias,Strand-Bias_>50%_P-value,Strand-Bias_>65%_P-value,Variant_Frequency,Variant_Nucleotide(s),Variant_P-Value_(approximate),,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,\nChr2_FT,Chr2,Chr2.bed,CDS,10000_ARHGAP15,GAAAGAATCATTAACAGTTAGAAGTTGATG-AAGTTTCAATAACAAGTGGGCACTGAGAGAAAG,55916421,56019336,55916483,56019399,63,64,1,forward,,,User,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,", header=TRUE, colClasses=c(rep("character", 24), rep("NULL", 41)), comment.char="", sep=",")
  Document_Name Sequence_Name Track_Name Type           Name
1       Chr2_FT          Chr2   Chr2.bed  CDS 10000_ARHGAP15
                                                        Sequence  Minimum Min_.with_gaps...  Maximum
1 GAAAGAATCATTAACAGTTAGAAGTTGATG-AAGTTTCAATAACAAGTGGGCACTGAGAGAAAG 55916421          56019336 55916483
  Max_.with_gaps. Length Length_.with_gaps. X._Intervals Direction Average.._Quality Coverage modified_by
1        56019399     63                 64            1   forward                                     User
  Polymorphism_Type Strand.Bias Strand.Bias_.50._P.va..lue Strand.Bias_.65._P.value Variant_Frequency
1                                                                                                    
  Variant_Nucleotide.s. Variant_P.Va..lue_.approximate.
1                                                      
If you know what your colClasses will be, then you can get missing values to be NA in the numeric columns automatically. You could also use the na.strings setting to accomplish this. You could also do some editing on the header to take out the illegal characters in the column names. (I didn't think I needed to be the one to do that though.)
read.table(text="Document_Name,Sequence_Name,Track_Name,Type,Name,Sequence,Minimum,Min_(with_gaps),Maximum,Max_(with_gaps),Length,Length_(with_gaps),#_Intervals,Direction,Average_Quality,Coverage,modified_by,Polymorphism_Type,Strand-Bias,Strand-Bias_>50%_P-value,Strand-Bias_>65%_P-value,Variant_Frequency,Variant_Nucleotide(s),Variant_P-Value_(approximate),,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
 Chr2_FT,Chr2,Chr2.bed,CDS,10000_ARHGAP15,GAAAGAATCATTAACAGTTAGAAGTTGATG-AAGTTTCAATAACAAGTGGGCACTGAGAGAAAG,55916421,56019336,55916483,56019399,63,64,1,forward,,,User,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,", header=TRUE, colClasses=c(rep("character", 24), rep("NULL", 41)), comment.char="", sep=",", na.strings="")
#------------------------------------------------------
  Document_Name Sequence_Name Track_Name Type           Name
1       Chr2_FT          Chr2   Chr2.bed  CDS 10000_ARHGAP15
                                                        Sequence  Minimum Min_.with_gaps...  Maximum
1 GAAAGAATCATTAACAGTTAGAAGTTGATG-AAGTTTCAATAACAAGTGGGCACTGAGAGAAAG 55916421          56019336 55916483
  Max_.with_gaps. Length Length_.with_gaps. X._Intervals Direction Average.._Quality Coverage modified_by
1        56019399     63                 64            1   forward              <NA>     <NA>          User
  Polymorphism_Type Strand.Bias Strand.Bias_.50._P.va..lue Strand.Bias_.65._P.value Variant_Frequency
1              <NA>        <NA>                       <NA>                     <NA>              <NA>
  Variant_Nucleotide.s. Variant_P.Va..lue_.approximate.
1                  <NA>                            <NA>