A Paul R said, have a look at Zoom. You can also use lsstack, which is a low-tech approach but surprisingly effective, compared to gprof.
Added: Since you clarified that you are running OpenGL at 33ms, my prior recommendation stands. In addition, what I personally have done in situations like that is both effective and non-intuitive. Just get it running with a typical or problematic workload, and just stop it, manually, in its tracks, and see what it's doing and why. Do this several times.
Now, if it only occasionally misbehaves, you would like to stop it only while it's misbehaving. That's not easy, but I've used an alarm-clock interrupt set for just the right delay. For example, if one frame out of 100 takes more than 33ms, at the start of a frame, set the timer for 35ms, and at the end of a frame, turn it off. That way, it will interrupt only when the code is taking too long, and it will show you why. Of course, one sample might miss the guilty code, but 20 samples won't miss it.