Here it goes:
Schema:
create table TableName 
( Source_ID varchar(100) not null,
 Env_ID varchar(100) not null,
 Record_ID  varchar(100),
 Item_Number varchar(100) not null, 
 Item_Description varchar(100), 
 Item_Class varchar(100)
)
alter table TableName add constraint PK_Env primary key (Env_ID, Source_ID, Item_Number)
insert into TableName (Source_ID,
                   Env_ID, 
                   Record_ID, 
                   Item_Number, 
                   Item_Description,
                   Item_Class)
values ('1','1','IM','0010000500K2#TI', 'BOLA B.1.6".5-1AC','KA')
insert into TableName (Source_ID,
                   Env_ID, 
                   Record_ID, 
                   Item_Number, 
                   Item_Description,
                   Item_Class)
values ('1','4','IM','0010000500K2#TI', 'BOLA B.1.6".5-1AC','KA')
Solution from: How can I remove duplicate rows?
;WITH cte
 AS (SELECT ROW_NUMBER() OVER (PARTITION BY Source_ID, Record_ID, Item_Number, Item_Description, Item_Class
                                   ORDER BY (Env_ID) desc) RN
     FROM   TableName WHERE Env_ID = '1' or Env_ID = '4')
DELETE FROM cte
WHERE  RN > 1
It checks all fields BUT Env_ID. Hope it helps and next time please: https://stackoverflow.com/help/how-to-ask