If you set your Form's AutoValidate mode to EnableAllowFocusChange, and presuming you have validating events hooked up to each of the controls inside your panel, something like this:
private void tb_Validating(object sender, CancelEventArgs e)
{
    TextBox tb = sender as TextBox;
    if (tb != null)
    {
        if (tb.Text == String.Empty)
        {
            errorProvider1.SetError(tb, "Textbox cannot be empty");
            e.Cancel = true;
        }
        else
            errorProvider1.SetError(tb, "");                    
    }
}
Then on the Click handler for your save button, you can just do this:
private void SaveButton_Click(object sender, EventArgs e)
{
    foreach (Control c in panel1.Controls)
       c.Focus();
    // If you want to summarise the errors
    StringBuilder errorSummary = new StringBuilder();
    foreach (Control c in panel1.Controls){
        String error = errorProvider1.GetError(c);
        if (error != String.Empty) 
            errorSummary.AppendFormat("{0}{1}", errorProvider1.GetError(c), Environment.NewLine);
    }
    if(errorSummary.Length>0)
        MessageBox.Show(errorSummary.ToString());
}
That will cause the validation to fire on each of the controls within the panel.