I am in an introductory Python class which is my first exposure to programming. Any help much appreciated. I am creating a piece of code that is to determine a linear regression function for a given set of inputs from a text file. I have defined the variable which contains the list of ordered pairs (x,y), as global pairs. However, I keep getting the error that pairs is not defined. I can't tweak any other parts of my code because this list is empty, causing the other lists I derived from this one to also be empty. I am genuinely stuck on this, I have looked for the answer on this site and others but I have not yet found the solution. 
This is some of the code I have:
#read values into tuple to seperate the spaces from X and Y values from the text file
#convert the tuple to a list containing (x,y) paris
    #the values are stored if we call the funciton
    #but the list of pairs doesn't seem to be global, it is empty when i just print(pairs)
def list_comprehension(in2):
    infile = open("in2",'r')
    global coordinates
    coordinates = (line.split() for line in infile)
    infile.close()
    global pairs
    pairs = [(float(x),float(y)) for x,y in coordinates]
    pairs.append(coordinates)
    return pairs 
#isolate x and y variables into seperate lists
    #same problem, the funciton operates fine
    #but the lists have nothing in them because pairs has nothing in it
X=[]
Y=[]
def isolate(X,Y):
    for (x,y) in pairs:
        X.append(x)
        Y.append(y)
    return X, Y
And the error is this:
 File "C:/Python34/python/Program 5/p5 draft function and values.py", line 47, in isolate
    for (x,y) in pairs:
NameError: name 'pairs' is not defined
 
     
     
     
     
    