Here I've got a data-frame consisted of 3264 normalized datapoint for 34 samples (3264 x 34). I have generated a ggplot using the following script:
cl21  <- geom_line(aes(y=CL021, col="CL"))
cl22  <- geom_line(aes(y=CL022, col="CL"))
cl24  <- geom_line(aes(y=CL024, col="CL"))
cl25  <- geom_line(aes(y=CL025, col="CL"))
cl27  <- geom_line(aes(y=CL027, col="CL"))
cl28  <- geom_line(aes(y=CL028, col="CL"))
cl30  <- geom_line(aes(y=CL030, col="CL"))
cl33  <- geom_line(aes(y=CL033, col="CL"))
cl35  <- geom_line(aes(y=CL035, col="CL"))
cl36  <- geom_line(aes(y=CL036, col="CL"))
cl37  <- geom_line(aes(y=CL037, col="CL"))
cl38  <- geom_line(aes(y=CL038, col="CL"))
cl39  <- geom_line(aes(y=CL039, col="CL"))
cl40  <- geom_line(aes(y=CL040, col="CL"))
ng172  <- geom_line(aes(y=NG172, col="NG"))
ng176  <- geom_line(aes(y=NG176, col="NG"))
ng178  <- geom_line(aes(y=NG178, col="NG"))
ng190  <- geom_line(aes(y=NG190, col="NG"))
ng191  <- geom_line(aes(y=NG191, col="NG"))
ng195  <- geom_line(aes(y=NG195, col="NG"))
ng218  <- geom_line(aes(y=NG218, col="NG"))
ng232  <- geom_line(aes(y=NG232, col="NG"))
ng244  <- geom_line(aes(y=NG244, col="NG"))
ng264  <- geom_line(aes(y=NG264, col="NG"))
ng285  <- geom_line(aes(y=NG285, col="NG"))
ng289  <- geom_line(aes(y=NG289, col="NG"))
ng299  <- geom_line(aes(y=NG299, col="NG"))
ng302  <- geom_line(aes(y=NG302, col="NG"))
ng306  <- geom_line(aes(y=NG306, col="NG"))
ng318  <- geom_line(aes(y=NG318, col="NG"))
ng320  <- geom_line(aes(y=NG320, col="NG"))
ng335  <- geom_line(aes(y=NG335, col="NG"))
ng338  <- geom_line(aes(y=NG338, col="NG"))
ng367  <- geom_line(aes(y=NG367, col="NG"))
gplot(normDF, aes(x=pos, y= value))+ cl21 +cl22  +cl24 +cl25 +cl27 +cl28 +cl30 +cl33 +cl35 +cl36 +cl37 +cl38 +cl39 +cl40 + ng172+ ng176+ ng178+ ng190+ ng191+ ng195+ ng218+ ng232+ ng244+ ng264+ ng285+ ng289+ ng299+ ng302+ ng306+ ng318+ ng320+ ng335+ ng338+ ng367  -> p
I tried to add customized tick marks along the x axis by defining breaks and labels:
p + xlab("FGD1 exons")+ ylab('Normalized Depth')+ scale_alpha_discrete(breaks= c("327","492","675","822","945","997","1098","1212","1380","1460","1620","1798","1968","2079","2542","2741","2936","3263"), labels= c("1","2","3","4","5","6","7","8","9","10","11","12","13","14","15","16","17","18"))+ theme(axis.text.x  = element_text(angle=90,vjust=0.5, size=15,hjust=1))
but ticks are not appearing on my plot! I was wondering if anyone here can advice me on this!
 
    
