This is a very common mistake.
The IN operator expect a list of values, but you are supplying a single value that happens to contain a list.
You should create a different parameter for each value in the EmployeeIds list.
Here is one way to do it:
string EmployeeIds = "'1231','1232','1233'";
var values = EmployeeIds.Split(',');
using(var command = new OleDbCommand())
{
    var sql = "SELECT [Employee Number], [Employee Name], [First In Time], [Last Out Time], [Total Work Hours] "+
              "FROM [Sheet0$A2:J] "+
              "WHERE [Employee Number] IN (";
    for(int i=0; i < values.Length; i++) 
    {
        // Please note that string interpolation will work only with c# 6 or later.
        // If you are working with vs 2013 or earlier, use string.Format instead.
        sql = $"{sql} @{i},";
        command.Parameters.Add($"@{i}", OleDbType.Int).Value = values[i].Trim(new char[] {'}); // You don't need the ' anymore since you are working with parameters now...
    }
    command.CommandText = sql.TrimEnd(',') +");";
    command.Connection = con;
    using(var reader = Command.ExecuteReader())
    {
        while(reader.Read())
        {
            // do your stuff with the data
        }
    }
}