I am using Scenebuilder and JavaFX 8.
I have a ComboBox in a VBox and I am trying to remove the ComboBox from the VBox when the Application starts on a simple condition. This is my attempt:
Main.java
public class Main extends Application {
    @Override
    public void start(Stage window) throws IOException {
        int QueryValue = 1;
        if (QueryValue == 1) {
            FXMLLoader loader=new FXMLLoader();
    Parent root = loader.load(getClass().getResource("/com/pos/view/login.fxml"));
    LoginController lc= (LoginController)loader.getController();
            lc.setInvisible();
            Scene scene = new Scene(root);
            window.setScene(scene);
            window.show();
        }
    }
    // ...
}
Controller class:LoginController.java
@FXML
private ComboBox<?> organization_combo;
@FXML
private  VBox vbox;
public  void setInvisible() {
    vbox.getChildren().remove(organization_combo);
}
I have added my controller class in FXML and also give fx:id for VBoxand ComboBox. The problem is whenever I call setInvisible()  I am getting NullPointerException. But my fxml is loading well when I am not calling that setInvisible() method. Can anyone help me? 
N.B: I have followed JavaFX - How to delete a specific Node from an AnchorPane and my problem is not matching the marked one.
Here is error message.
Caused by: java.lang.RuntimeException: Exception in Application start method
    at javafx.graphics/com.sun.javafx.application.LauncherImpl.launchApplication1(Unknown Source)
    at javafx.graphics/com.sun.javafx.application.LauncherImpl.lambda$launchApplication$2(Unknown Source)
    at java.base/java.lang.Thread.run(Unknown Source)
Caused by: java.lang.NullPointerException
    at com.pos.view.LoginController.setInvisible(LoginController.java:45)
    at com.pos.main.Main.start(Main.java:26)
    at javafx.graphics/com.sun.javafx.application.LauncherImpl.lambda$launchApplication1$9(Unknown Source)
    at javafx.graphics/com.sun.javafx.application.PlatformImpl.lambda$runAndWait$11(Unknown Source)
    at javafx.graphics/com.sun.javafx.application.PlatformImpl.lambda$runLater$9(Unknown Source)
    at java.base/java.security.AccessController.doPrivileged(Native Method)
    at javafx.graphics/com.sun.javafx.application.PlatformImpl.lambda$runLater$10(Unknown Source)
    at javafx.graphics/com.sun.glass.ui.InvokeLaterDispatcher$Future.run(Unknown Source)
    at javafx.graphics/com.sun.glass.ui.win.WinApplication._runLoop(Native Method)
    at javafx.graphics/com.sun.glass.ui.win.WinApplication.lambda$runLoop$3(Unknown Source)
 
    