EDIT: Since hek2mgl sir mentioned in case you need to remove continuous similar lines then try following.
Let's say following is Input_file:
cat Input_file
chr1    109472560   109472561   -4732   CLCC1
chr1    109472560   109472561   -4732   CLCC1
chr1    109477498   109477499   206 CLCC1
chr1    109477498   109477499   206 CLCC1
chr1    109477498   109477499   206 CLCC1
chr1    109472560   109472561   -4732   CLCC1
chr1    109477498   109477499   206 CLCC1
chr1    109472560   109472561   -4732   CLCC1
Run following code now:
awk 'prev!=$0;{prev=$0}'  Input_file
Output will be as follows.
chr1    109472560   109472561   -4732   CLCC1
chr1    109477498   109477499   206 CLCC1
chr1    109472560   109472561   -4732   CLCC1
chr1    109477498   109477499   206 CLCC1
chr1    109472560   109472561   -4732   CLCC1
The following snippet will remove all duplicate lines, not only repeating lines
awk '!a[$0]++'  Input_file
Append > output_file to above command in case you want to take output into a separate file.
Explanation: Adding explanation for above code now. This is only for explanation purposes for running code use above mentioned one only.
awk '
!a[$0]++      ##Checking condition here if current line is present in array a index or NOT, if not then increase its value by 1.
              ##So that next time it will make condition as FALSE, since we need to have only unique lines.
              ##awk works on method of condition and action, so if condition is TRUE it will do some action mentioned by programmer.
              ##Here I am not mentioning action so by default print of current line will happen, whenever condition is TRUE.
'  Input_file  ##mentioning Input_file name here.