Your use of global is not correct when you say global pot += global aibet
Using the global keyword inside a lower scope (like a function or a class) tells python to use the global version of that variable and not the local version. (You can access a dictionary of the global variables using globals()). 
This means that in every function you modify pot and aibet, you need to declare the variable as global before you use it. 
This can get messy and the use of global variables like this is frowned upon unless absolutely necessary (which for your case it is NOT necessary).
So first, in order to fix your issue, you will need to:
- Add this to your AIbetfunction before you use either variable. Remember, this is telling the interpreter to use the global version of these variables instead of the local version.
global pot
global aibet
- Add this to your AIspinfunction. You usedeadin theifstatement in the global scope later in your code, sodeadalso needs to beglobal.
global dead
- I'm speculating that your if dead == deathspin:statement should be indented and inside theAIspinfunction. If this is the case, the only changes you have to make is to add global declarations forpot,aibet, andaibudgetlike you did fordeadand replaceglobal pot += global aibetwithpot += aibet. Doing so gives us this for yourAIspinfunction:
def AIspin():
    global dead
    global pot
    global aibet
    global aibudget
    print()
    print("The AI spins the chamber...")
    print("...And pulls the trigger.")
    print()
    dead = 6
    if dead == deathspin:
        print("The AI lands on the bad cartridge")
        print()
        pot += aibet
        aibudget -= aibet
        print("The pot is currently: $",pot,sep="")
        print("The AI has a hand of $",aibudget,".",sep="")
But don't you think this looks bad and is riddled with unnecessary global statements? Instead, adapt a class based approach where you don't need to pass around so many global variables. It would look like this:
import random
class RussianRoulette:
    def __init__(self):
        self.pot = 0
        self.playerbudget = 500
        self.aibudget = 500
        self.playerbet = 0
        self.aibet = 10
        self.deathspin = 6
        self.dead = 6
    def ai_bet(self):
        print()
        self.aibet = random.randint(50,250)
        print(f"The AI bets: ${self.aibet:.2f}")
        return
    def ai_spin(self):
        print()
        print("The AI spins the chamber...")
        print("...And pulls the trigger.")
        print()
        if self.dead == self.deathspin:
            print("The AI lands on the bad cartridge")
            print()
            self.pot += self.aibet
            self.aibudget -= self.aibet
            print(f"The pot is currently: ${self.pot:.2f}")
            print(f"The AI has a hand of ${self.aibudget:.2f}")
        return
You would then use this class like this:
game = RussianRoulette()
game.ai_spin()
game.ai_spin()
game.ai_spin()
Which outputs:
The AI spins the chamber...
...And pulls the trigger.
The AI lands on the bad cartridge
The pot is currently: $10.00
The AI has a hand of $490.00
The AI spins the chamber...
...And pulls the trigger.
The AI lands on the bad cartridge
The pot is currently: $20.00
The AI has a hand of $480.00
The AI spins the chamber...
...And pulls the trigger.
The AI lands on the bad cartridge
The pot is currently: $30.00
The AI has a hand of $470.00
Summary: Avoid using global and use a class approach if possible. You can pack "shared" variables into your class instance. The class instance acts like a container for your variables (in your case). We can access the variables anywhere within the class' methods by prepending the variable name with self (assuming that's what you named the first argument). 
Consider reading:
- Easy to follow tutorial on python classes
- Official documentation on python classes