To display subprocess' output in a GUI while it is still running, a portable stdlib-only solution that works on both Python 2 and 3 has to use a background thread:
#!/usr/bin/python
"""
- read output from a subprocess in a background thread
- show the output in the GUI
"""
import sys
from itertools import islice
from subprocess import Popen, PIPE
from textwrap import dedent
from threading import Thread
try:
    import Tkinter as tk
    from Queue import Queue, Empty
except ImportError:
    import tkinter as tk # Python 3
    from queue import Queue, Empty # Python 3
def iter_except(function, exception):
    """Works like builtin 2-argument `iter()`, but stops on `exception`."""
    try:
        while True:
            yield function()
    except exception:
        return
class DisplaySubprocessOutputDemo:
    def __init__(self, root):
        self.root = root
        # start dummy subprocess to generate some output
        self.process = Popen([sys.executable, "-u", "-c", dedent("""
            import itertools, time
            for i in itertools.count():
                print("%d.%d" % divmod(i, 10))
                time.sleep(0.1)
            """)], stdout=PIPE)
        # launch thread to read the subprocess output
        #   (put the subprocess output into the queue in a background thread,
        #    get output from the queue in the GUI thread.
        #    Output chain: process.readline -> queue -> label)
        q = Queue(maxsize=1024)  # limit output buffering (may stall subprocess)
        t = Thread(target=self.reader_thread, args=[q])
        t.daemon = True # close pipe if GUI process exits
        t.start()
        # show subprocess' stdout in GUI
        self.label = tk.Label(root, text="  ", font=(None, 200))
        self.label.pack(ipadx=4, padx=4, ipady=4, pady=4, fill='both')
        self.update(q) # start update loop
    def reader_thread(self, q):
        """Read subprocess output and put it into the queue."""
        try:
            with self.process.stdout as pipe:
                for line in iter(pipe.readline, b''):
                    q.put(line)
        finally:
            q.put(None)
    def update(self, q):
        """Update GUI with items from the queue."""
        for line in iter_except(q.get_nowait, Empty): # display all content
            if line is None:
                self.quit()
                return
            else:
                self.label['text'] = line # update GUI
                break # display no more than one line per 40 milliseconds
        self.root.after(40, self.update, q) # schedule next update
    def quit(self):
        self.process.kill() # exit subprocess if GUI is closed (zombie!)
        self.root.destroy()
root = tk.Tk()
app = DisplaySubprocessOutputDemo(root)
root.protocol("WM_DELETE_WINDOW", app.quit)
# center window
root.eval('tk::PlaceWindow %s center' % root.winfo_pathname(root.winfo_id()))
root.mainloop()
The essence of the solution is:
- put the subprocess output into the queue in a background thread
- get the output from the queue in the GUI thread.
i.e., call process.readline() in the background thread -> queue -> update GUI label in the main thread. Related kill-process.py (no polling -- a less portable solution that uses event_generate in a background thread).