There are already several good answers here, but none discuss the general concept of "truthy" and "falsy" expressions in Python.
In Python, truthy expressions are expression that return True when converted to bool, and falsy expressions are expressions that return False when converted to bool. (Ref: Trey Hunner's regular expression tutorial; I'm not affiliated with Hunner, I just love his tutorials.)
Falsy stuff:
What's important here is that 0, 0.0, [], None and False are all falsy.
When used in an if statement, they will fail the test, and they will pass the test in an if not statement.
Truthy stuff:
Non-zero numbers, non-empty lists, many objects (but read @tdelaney's answer for more details here), and True are all truthy, so they pass if and fail if not tests.
Equality tests
When you use equality tests, you're not asking about the truthiness of an expression, you're asking whether it is equal to the other thing you provide, which is much more restrictive than general truthiness or falsiness.
EDIT: Additional references
Here are more references on "Truthy" and "Falsy" values in Python: