The gap property only applies to flex-parents, not flex-items (flex-parents use it to control the gap between their child flex-items).
- (The
gap property also applies to display: grid;, but that's off-topic in this QA)
To adjust the spacing of flex-items use an overridden margin property instead.
For flex-direction: horizontal you'll want to set margin-left and margin-right (or margin-inline-start and margin-inline-end).
For flex-direction: vertical you'll want to set margin-top and margin-bottom (or margin-block-start and margin-block-end).
To have a larger effective gap then set any positive margin value.
- e.g. with
gap: 5px and margin-left: 1px then the effective gap to the left will be 6px.
To have a smaller effective gap then set a negative margin value.
- e.g. with
gap: 5px and margin-left: -1px then the effective gap to the left will be 4px.
This approach won't work for items with margin: auto, but that's okay because gap: isn't useful with auto margins anyway.
Don't forget that you can also use calc and --custom-properties to make the CSS easier to read, and to mix units (e.g. 3.2em margin with a 10px effective gap adjustment).
In your case, there's 20px on either side of your items, so applying a margin-left of -15px will give you a 5px left gap, and a margin-right of -15px will give you a 5px right gap.
Which looks like this:

Like so:
.container{
display: flex;
gap: 20px;
}
.items{
width: 100px;
height: 100px;
background: red;
}
.item-4 {
background: blue;
margin-left: -15px; /* Adapt 20px gap to 5px */
margin-right: -15px; /* Adapt 20px gap to 5px */
}
<div class="container">
<div class="items item-1"></div>
<div class="items item-2"></div>
<div class="items item-3"></div>
<div class="items item-4"></div>
<div class="items item-5"></div>
<div class="items item-6"></div>
</div>