I have implemented azure ad authentication in SPA app and validating the token using OWIN start.cs. it is working fine in local environment. below code used for token validation:
    app.UseWindowsAzureActiveDirectoryBearerAuthentication(new WindowsAzureActiveDirectoryBearerAuthenticationOptions
    {
        Tenant = tenant,
        TokenValidationParameters = new TokenValidationParameters
        {
            //ValidAudiences = new[] { Audience },
            ValidAudience = validateAudience,
            ValidateIssuer = false,
            ValidIssuers = new[] { ValidIssuers }
        }
    });
when I deployed this published code on prem server and run web api it throws error as mentioned in below screen shot. I have used Microsoft.Owin.Security.ActiveDirectory version 4.2.2.0,  

can anyone help on this ?
 
    