How to separate 2 of the columns from the dataframe and create a different dataframe with the 2

Question

df= col1 col2 col3 col4.... col300 
     21    31  43  64         21

I want to get to

df= col1 col2  col5.... col300 
     21    31    27       21 



df2=  col3  col4
     43     64

How can I do that with a large dataset? I want split the dataframe, or drop 2 of the columns as a separate dataframe?