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I'm trying to do a dotplot with my KEEG analysis result for pathways, however, almost every script that has about it needs an FC value to perform the analysis. I don't have this value, because my data came from my miRNA analysis, which generated the targets and then I was able to establish the pathways. I would like to put the routes in a dotplot like this one in the imagem enter image description here

These are examples of my data:

enter image description here

I followed a script from a colleague here:

ggplot(df, aes(x=rf,y=pathway_term)) + 
  geom_point(aes(color=pval,size=gene)) + 
  scale_color_gradientn(colours = rainbow(5)) + 
  labs(x='Rich Factor', y=NULL, color='P-value',size='Gene\nnumber' ) + 
  theme( axis.title = element_text(face='bold'), axis.text = element_text (face='bold') )

However, the error appears: Error: Discrete value supplied to continuous scale Could someone help me solve my problem?

MrFlick
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Luiza Veiga
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  • Please share your data in a [reproducible format](https://stackoverflow.com/questions/5963269/how-to-make-a-great-r-reproducible-example). Please [do not post code or data in images](https://meta.stackoverflow.com/q/285551/2372064) – MrFlick Mar 28 '23 at 21:37
  • term enrichment pvalue count EGFR tyrosine kinase inhibitor resistance 2,5 1,1E-09 20 MAPK signaling pathway 4,9 2,6E-09 39 ErbB signaling pathway 2,4 0,000000027 19 Proteoglycans in cancer 3,8 0,00000003 30 MicroRNAs in cancer 4,8 0,000000038 38 PI3K-Akt signaling pathway 5,1 0,00000014 40 Focal adhesion 3,4 0,00000095 27 Pathways in cancer 6,2 0,000002 49 Signaling pathways regulating pluripotency of stem cells 2,7 0,0000053 21 Glioma 1,8 0,000024 14 – Luiza Veiga Mar 29 '23 at 16:03

0 Answers0